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Environmental Salmonella PCR Fluxergy Test Kit

A simple and cost-effective test to ensure the veterinary hospital environment is protected from Salmonella.

  • Improve Biosecurity and boost client confidence to mitigate risk of hospital acquired infection (HAI).

  • Simple workflow after enrichment by using a sensitive and specific RT-PCR test.

  • Fast results in < 1 hour. Reference Laboratory PCR can take 3-5 days.

  • Confirm disinfection protocol and increase speed of stall turnover with on-site Salmonella PCR testing with results the next morning.

No Fluxergy products have been cleared or approved by the USDA for veterinary diagnostic use. Fluxergy’s current veterinary product offerings are limited to
individual laboratory components for use by experienced professional users in developing their own laboratory tests for research or animal use.

Who We Work With

Clinically Validated to Ensure Performance

Analytical Reactivity
This study is designed to assess the performance of Fluxergy PCR Salmonella Assay with Salmonella serotypes. Specific serotypes were selected due to their clinical relevance. A total of 20 serotypes were tested on the Fluxergy platform.


Salmonella spp. was serially diluted in negative pooled environmental swab matrix down to 1CFU/mL. 1mL of 1CFU/mL was spiked into BD BBL™ Mycoflask™ Selenite Cystine Broth Prepared Media (BD 297711). Culture was incubated (GBiosciences Incubator Shaker overnight at 37C for 16 hours.) The LOD of 1 CFU/mL was confirmed by detecting > 95% of 20 replicates tested.

Cross-Reactivity Testing
Cross Reactivity of the Fluxergy Environmental Salmonella Test Kit was evaluated by wet-lab testing using the Fluxergy Analyzer. A panel of 23 microorganisms were tested at a concentration of 1X105 copies/mL. All organisms tested negative in triplicate.

Use Cases: Save Cost with Faster Stall Turnover

On-site testing allows for quick answers when dealing with suspect cases:

  • ICU stalls (Prior to patient admit )
  • Surgical suites (Post-colic surgery)
  • Horse trailers (Post-travel for stress-induced shedding)
  • Routine screening of probable practice locations
  • Clinics or referral hospitals
  • Ambulatory practices
  • Veterinary teaching hospitals

Frequently Asked Questions

Salmonella  can be isolated from a mat located in the colic surgery room. 18 of the 33 cases in this outbreak had surgery, and it is possible that some cases were infected during surgery. In other outbreaks in teaching hospitals, Salmonella has been isolated from a range of sites, including horse feeders and waterers, floor drains, stalls and stall mats, operating tables, recovery room mats and office floors.

The risk of Salmonella shedding more than doubled in patients with abdominal surgery versus non-surgical patients. Patients who underwent abdominal surgery were also eight times more likely to contract a hospital-acquired Salmonella infection.

To reduce fomite risk of Salmonella contamination, test nasogastric tubing and buckets, manure pitchforks, and ambulatory vehicle floor mats, tires and steering wheels, and tractor tires. Salmonella has been frequently isolated from reflux samples, so the risk of Salmonella environmental contamination and hospital-acquired infections is increased. Improved environmental monitoring allows for earlier detection and isolation, reducing environmental contamination.

Adult horses that had been treated with antimicrobial drugs prior to hospitalization were 3.09 times as likely to be shedding Salmonella organisms as were adult horses that had not been treated with antimicrobial drugs prior to hospitalization.

  1. Ward, M. P., Brady, T. H., Couëtil, L. L., Liljebjelke, K., Maurer, J. J., & Ching, C. W. (2005). Investigation and control of an outbreak of salmonellosis caused by multidrug-resistant Salmonella typhimurium in a population of hospitalized horses. Veterinary Microbiology, 107(3–4), 233–240. https://doi.org/10.1016/J.VETMIC.2005.01.019
  2. Ernst, N. S., Hernandez, J. A., MacKay, R. J., Brown, M. P., Gaskin, J. M., Nguyen, A. D., Giguere, S., Colahan, P. T., Troedsson, M. R., Haines, G. R., Addison, I. R., & Miller, B. J. (2004). Risk factors associated with fecal Salmonella shedding among hospitalized horses with signs of gastrointestinal tract disease. Journal of the American Veterinary Medical Association, 225(2), 275–281. https://doi.org/10.2460/JAVMA.2004.225.275
  3. Ekiri, A. B., MacKay, R. J., Gaskin, J. M., Freeman, D. E., House, A. M., Giguère, S., Troedsson, M. R., Schuman, C. D., Von Chamier, M. M., Henry, K. M., & Hernandez, J. A. (2009). Epidemiologic analysis of nosocomial Salmonella infections in hospitalized horses. Journal of the American Veterinary Medical Association, 234(1), 108–119. https://doi.org/10.2460/JAVMA.234.1.10
  4. Rothers, K. L., Hackett, E. S., Mason, G. L., & Nelson, B. B. (2020). Atypical Salmonellosis in a Horse: Implications for Hospital Safety. Case Reports in Veterinary Medicine, 2020. https://doi.org/10.1155/2020/7062408
  5. Dallap Schaer, B. L., Aceto, H., Caruso, M. A., & Brace, M. A. (2012). Identification of Predictors of Salmonella Shedding in Adult Horses Presented for Acute Colic. Journal of Veterinary Internal Medicine, 26(5), 1177–1185. https://doi.org/10.1111/J.1939-1676.2012.00984.X

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