Environmental Salmonella PCR Fluxergy Test Kit
A simple and cost-effective test to ensure the veterinary hospital environment is protected from Salmonella.
Improve Biosecurity and boost client confidence to mitigate risk of hospital acquired infection (HAI).
Simple workflow after enrichment by using a sensitive and specific RT-PCR test.
Fast results in < 1 hour. Reference Laboratory PCR can take 3-5 days.
Confirm disinfection protocol and increase speed of stall turnover with on-site Salmonella PCR testing with results the next morning.
The Fluxergy Analyzer system, which utilizes state-of-the-art PCR and microfluidics technology, has been shown to accurately identify Salmonella in ~1 hour.
1. Swab Stall or Drain
2. Enrich Sample Overnight
3. Run PCR Results in 1 Hour
Salmonella is a leading cause of enterocolitis in susceptible horses. Clinical cases range from asymptomatic to acute and severe diarrhea to death. Further, it is possible for disease outbreaks and population epidemics to occur depending on the organism, host and degree of exposure. It is also possible for subclinical shedders to endanger the health of hospitalized patients and clinical personnel.
It is thus recommended that PCR be used for rapid and specific pathogen detection to maintain biosecurity. Microbiological culture is typically used in tandem as standard of care due to only small concentrations of Salmonella spp. commonly being present.
This Fluxergy assay targets a conserved gene responsible for invasion of epithelial cells by all pathogenic strains of Salmonella. Fluxergy further offers a 12-hour incubation buffer that can be used with the Fluxergy Salmonella spp. assay to increase test sensitivity and ensure absence or presence of pathogen.
Who We Work With
Clinically Validated to Ensure Performance
This study is designed to assess the performance of Fluxergy PCR Salmonella Assay with Salmonella serotypes. Specific serotypes were selected due to their clinical relevance. A total of 20 serotypes were tested on the Fluxergy platform.
Salmonella spp. was serially diluted in negative pooled environmental swab matrix down to 1CFU/mL. 1mL of 1CFU/mL was spiked into BD BBL™ Mycoflask™ Selenite Cystine Broth Prepared Media (BD 297711). Culture was incubated (GBiosciences Incubator Shaker overnight at 37C for 16 hours.) The LOD of 1 CFU/mL was confirmed by detecting > 95% of 20 replicates tested.
Cross Reactivity of the Fluxergy Environmental Salmonella Test Kit was evaluated by wet-lab testing using the Fluxergy Analyzer. A panel of 23 microorganisms were tested at a concentration of 1X105 copies/mL. All organisms tested negative in triplicate.
|Test Type||Direct RT-PCR|
|Time to Result||~1 hour|
|Sample Preparation||16 hours for enrichment, ~3 min from reagent thaw|
|Sample Type||Environmental Sponge (Included)|
|Compatible Enrichments||Selenite Cysteine Broth (Included)|
|Required Sample Volume||14 uL of Selenite Cysteine Broth|
|Storage||(Flux Card) 10° to 30°C, (Flux Reaction Mix) -10° to -30°C|
|Gene Target||Salmonella Spp. InvA|
|LOD||1 CFU / mL Post 16-hour Enrichment|
|Reader Compatibility||Fluxergy Analyzer|
|Analyzer Size||26.19 cm x 13.13 cm x 25.80 cm|
|Analyzer Weight||6.8 kg|
|Power Input||12VDC, 7A|
|Operating Temperature||15°- 30°C|
|Storage Temperature||15°- 30°C|
|Operating Humidity||10% – 85%|
Computer Reqs. (Recommended)
OS must be 64-bit, Windows 10+. Intel i5 2.5GHz or equivalent, 8GB DDR4 RAM, HDD: 250 GB, Screen 1080p, USB: 2×2.0 port, Networking: Ethernet port
Use Cases: Save Cost with Faster Stall Turnover
On-site testing allows for quick answers when dealing with suspect cases:
- ICU stalls (Prior to patient admit )
- Surgical suites (Post-colic surgery)
- Horse trailers (Post-travel for stress-induced shedding)
- Routine screening of probable practice locations
- Clinics or referral hospitals
- Ambulatory practices
- Veterinary teaching hospitals
Frequently Asked Questions
Salmonella can be isolated from a mat located in the colic surgery room. 18 of the 33 cases in this outbreak had surgery, and it is possible that some cases were infected during surgery. In other outbreaks in teaching hospitals, Salmonella has been isolated from a range of sites, including horse feeders and waterers, floor drains, stalls and stall mats, operating tables, recovery room mats and office floors.
The risk of Salmonella shedding more than doubled in patients with abdominal surgery versus non-surgical patients. Patients who underwent abdominal surgery were also eight times more likely to contract a hospital-acquired Salmonella infection.
To reduce fomite risk of Salmonella contamination, test nasogastric tubing and buckets, manure pitchforks, and ambulatory vehicle floor mats, tires and steering wheels, and tractor tires. Salmonella has been frequently isolated from reflux samples, so the risk of Salmonella environmental contamination and hospital-acquired infections is increased. Improved environmental monitoring allows for earlier detection and isolation, reducing environmental contamination.
Adult horses that had been treated with antimicrobial drugs prior to hospitalization were 3.09 times as likely to be shedding Salmonella organisms as were adult horses that had not been treated with antimicrobial drugs prior to hospitalization.
- Ward, M. P., Brady, T. H., Couëtil, L. L., Liljebjelke, K., Maurer, J. J., & Ching, C. W. (2005). Investigation and control of an outbreak of salmonellosis caused by multidrug-resistant Salmonella typhimurium in a population of hospitalized horses. Veterinary Microbiology, 107(3–4), 233–240. https://doi.org/10.1016/J.VETMIC.2005.01.019
- Ernst, N. S., Hernandez, J. A., MacKay, R. J., Brown, M. P., Gaskin, J. M., Nguyen, A. D., Giguere, S., Colahan, P. T., Troedsson, M. R., Haines, G. R., Addison, I. R., & Miller, B. J. (2004). Risk factors associated with fecal Salmonella shedding among hospitalized horses with signs of gastrointestinal tract disease. Journal of the American Veterinary Medical Association, 225(2), 275–281. https://doi.org/10.2460/JAVMA.2004.225.275
- Ekiri, A. B., MacKay, R. J., Gaskin, J. M., Freeman, D. E., House, A. M., Giguère, S., Troedsson, M. R., Schuman, C. D., Von Chamier, M. M., Henry, K. M., & Hernandez, J. A. (2009). Epidemiologic analysis of nosocomial Salmonella infections in hospitalized horses. Journal of the American Veterinary Medical Association, 234(1), 108–119. https://doi.org/10.2460/JAVMA.234.1.10
- Rothers, K. L., Hackett, E. S., Mason, G. L., & Nelson, B. B. (2020). Atypical Salmonellosis in a Horse: Implications for Hospital Safety. Case Reports in Veterinary Medicine, 2020. https://doi.org/10.1155/2020/7062408
- Dallap Schaer, B. L., Aceto, H., Caruso, M. A., & Brace, M. A. (2012). Identification of Predictors of Salmonella Shedding in Adult Horses Presented for Acute Colic. Journal of Veterinary Internal Medicine, 26(5), 1177–1185. https://doi.org/10.1111/J.1939-1676.2012.00984.X