Equine Herpesvirus 1 (EHV-1) PCR Fluxergy Assay
Rapid and clinically-relevant detection of EHV-1 from an equine nasal swab sample*
- Rapid Test Results: Fast, accurate diagnostics that can improve patient diagnoses.
- Stall-side care with minimal hands-on time per sample: Test before introducing a horse to a population or for fever of unknown origin (FUO).
- Give your clients answers and peace of mind within 1 hour from sample collection.
- Ideal for show grounds, equine facilities, boarding barns, or breeding farms that are concerned with respiratory pathogens.
*No Fluxergy products have been cleared or approved by the USDA for veterinary diagnostic use. Fluxergy’s current veterinary product offerings are limited to individual laboratory components for use by experienced professional users in developing their own laboratory tests for research or animal use

Validated against clinical samples
Method Comparison
Our study design tests the ability of the Fluxergy PCR EHV-1 Assay to detect contrived, near-LoD samples in individually collected equine nasal swab matrix samples. The nasal swab samples were collected and confirmed as negatives by PCR (validated commercial EHV-1 Test). Contrived samples included the following: 2x LoD and above with Equine Herpesvirus 1 Virus USDA strain (# 045-EDV). Native (Negative) and contrived (Positive) samples were tested with Fluxergy Assay PCR EHV-1 Test.
LOD
The LoD of the Fluxergy Assay PCR EHV-1 Test was determined using enumerated Equine Herpesvirus 1 Virus USDA strain (# 045-EDV). The viral stock was serially diluted to 0.028 copies/μL within pooled negative equine nasal matrix. A third LoD confirmation was tested at 500 copies/mL with 20/20 samples detected (LoD of 500 copies/mL).
Interference Testing
Potentially interfering substances commonly found in the equine upper respiratory system were tested for assay interference using samples containing EHV-1 045-EDV strain at 3x LoD. Testing was performed in triplicate and no interference was observed.
~3 min from reagent thaw, No extraction required
gB and gD
(10.31 inches x 5.16 inches x 10.16 inches)
A veterinary tool for every Equine facility
On-site testing allows for you to initially prevent spread and react when outbreaks occur:
- Facilities and Barns
- Clinics & Referral Hospitals
- Ambulatory Practices
- Veterinary Teaching Hospitals
- Shows
- Racetracks
FAQs
In recent years, EHV-1 outbreaks at local and FEI shows have had drastic consequences, including numerous horses lost, dozens of show cancellations, and strict quarantines. Because some sectors of the equine community compete throughout the season, multiple shows, barns, and geographic regions can be at risk within a few days.
Yes, EHV-1 has been identified on wood shavings, leather, and other supplies for at least 48 hours, even on items stored outside or in cool temperatures.These findings emphasize the need for increased biosecurity, particularly within high-volume equine-related facilities.
Multiple outbreaks have occurred due to the arrival or return of horses, including clinically healthy horses. Incorporating EHV-1 monitoring–especially for horses traveling from high-risk areas or events–better protects local and international equine populations.
Equine Herpesvirus 1 (EHV-1) is a common and burdensome DNA virus in horse populations globally. It is highly contagious and spreads through direct horse-to-horse contact or indirect contact e.g. contaminated hands, equipment. Moreover, the neurologic form of the disease is particularly troublesome due to associated degrees of paralysis and is seen in approximately 10% of infected horses.
Current diagnostic testing requires use of PCR for specific diagnosis as clinical signs of EHV-1 mimic that of other respiratory pathogens and neurological diseases.
Due to the different presentations of EHV-1, correct and timely testing helps improve biosecurity.
Still have questions?
Testimonials
"I have conducted research on the Fluxergy system for the past 3 years. Having the capability to have a patient-side, reliable, sensitive, and cost-effective molecular detection instrument can be a tremendous improvement in the diagnostic field. Fluxergy’s advanced technology can potentially be used on clinical and subclinical horses in order to allow for proper treatment and timely biosecurity. Fluxergy’s novel platform can turn a 1 to 3-day turnaround time into a 1-hour processing time."

Dr. Nicola Pusterla
UC Davis School of Veterinary Medicine
"It is great to have the Fluxergy PCR platform on-site. The quick turnaround time makes a huge difference for our clients and patients, and we anticipate that we will be able to use this technology in many different aspects of equine veterinary medicine. It has the potential to rapidly confirm viral cases in densely populated equine areas, which will significantly improve disease management strategies. Overall, we are extremely pleased with this technology."
Peter Heidmann
Head of Internal Medicine Palm Beach Equine
“I learned of Fluxergy’s technology when I was at Boehringer Ingelheim, and I was impressed about the opportunity for this point-of-care testing platform. I am happy to be involved on Fluxergy’s advisory board where I can bring my 20+ years of animal health and R&D experience. One of the big advantages of Fluxergy’s technology is that it is very easy to use. When veterinarians see horses with high fevers, its important that they can understand quickly what is going on.”

Dr. Randolph Seidler, VMD, MBA
Boehringer Ingelheim



