Our study design tests the ability of the Fluxergy PCR EHV-1 Assay to detect contrived, near-LoD samples in individually collected equine nasal swab matrix samples. The nasal swab samples were collected and confirmed as negatives by PCR (validated commercial EHV-1 Test). Contrived samples included the following: 2x LoD and above with Equine Herpesvirus 1 Virus USDA strain (# 045-EDV). Native (Negative) and contrived (Positive) samples were tested with Fluxergy Assay PCR EHV-1 Test.
The LoD of the Fluxergy Assay PCR EHV-1 Test was determined using enumerated Equine Herpesvirus 1 Virus USDA strain (# 045-EDV). The viral stock was serially diluted to 0.028 copies/μL within pooled negative equine nasal matrix. A third LoD confirmation was tested at 500 copies/mL with 20/20 samples detected (LoD of 500 copies/mL).
Potentially interfering substances commonly found in the equine upper respiratory system were tested for assay interference using samples containing EHV-1 045-EDV strain at 3x LoD. Testing was performed in triplicate and no interference was observed.
A veterinary tool for every Equine facility
On-site testing allows for you to initially prevent spread and react when outbreaks occur:
- Facilities and Barns
- Clinics & Referral Hospitals
- Ambulatory Practices
- Veterinary Teaching Hospitals
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HVRe Influenza / RSV / SARS-CoV-2 Respiratory Panel
Streptococcus equi ssp. equi
Dr. Lucan Witzkowski, DVM, PhD
Warsaw University of Life Sciences
Dr. Nicola Pusterla
UC Davis School of Veterinary Medicine
Head of Internal Medicine Palm Beach Equine
Dr. Randolph Seidler, VMD, MBA