fluxergy strep equi equi

Equine -> EHV-1

Equine Herpesvirus 1 (EHV-1) Fluxergy Test Kit

Rapid and clinically-relevant detection of EHV-1 from an equine nasal swab sample.

  • Rapid Test Results: Fast, accurate diagnostics that can improve patient diagnoses.

  • Stall-side care with minimal hands-on time per sample: Test before introducing a horse to a population or for fever of unknown origin (FUO).

  • Give your clients answers and peace of mind within 1 hour from sample collection.

  • Ideal for show grounds, equine facilities, boarding barns, or breeding farms that are concerned with respiratory pathogens.
Pre-Order with fluxergy

No Fluxergy products have been cleared or approved by the USDA for veterinary diagnostic use. Fluxergy’s current veterinary product offerings are limited to
individual laboratory components for use by experienced professional users in developing their own laboratory tests for research or animal use.

OVERVIEW

SPECIFICATIONS

USE CASES

FAQ

Testimonials

RESOURCES

About Equine Herpesvirus 1 (EHV-1)

Equine Herpesvirus 1 (EHV-1) is a common and burdensome DNA virus in horse populations globally. It is highly contagious and spreads through direct horse-to-horse contact or indirect contact e.g. contaminated hands,  equipment. Moreover, the neurologic form of the disease is particularly troublesome due to associated degrees of paralysis and is seen in approximately 10% of infected horses. 

Current diagnostic testing requires use of PCR for specific diagnosis as clinical signs of EHV-1 mimic that of other respiratory pathogens and neurological diseases. 

Due to the different presentations of EHV-1, correct and timely testing helps improve biosecurity.

View Veterinary Consensus Statement
julia horse

Who We Work With

socal equine
22-05-10-website-logos-halton
idaho equine
22-05-10-website-logos-brazos
signature equine
amwell data services
uc davis veterinary hospital equine
Previous
Next

Validated against Clinical Samples

Method Comparison
The study design will test the ability of the Fluxergy PCR EHV-1 Assay to detect contrived, near LoD samples in
individually collected equine nasal swab matrix samples. The nasal swab samples will be collected and confirmed as
as negatives by PCR (Validated commercial S. equi Test). Contrived samples included the following concentrations:
2x LoD and above with Equine herpesvirus 1 virus USDA strain (# 045-EDV). Native (Negative) and contrived
(Positive) samples will be tested with Fluxergy Assay PCR EHV-1 Test.

LOD
The LoD of the Fluxergy Assay PCR EHV-1 Test was performed with enumerated Equine herpesvirus 1 virus USDA
strain (# 045-EDV). The virus stock was serially diluted to 0.028 copies/μL within pooled negative equine nasal swab
matrix. Third LoD confirmation was tested at 1000 copies/mL with 20/20 tested (LoD of 1000 copies/mL).

Interference Testing
The following potentially interfering substances from equine upper respiratory system were tested for assay
interference using samples containing EHV1 045-EDV strain at 3x LoD. Testing was performed in triplicate at the
indicted levels. No interference was observed.

View Full Study
Test TypePCR, Direct PCR
Time to Result ~55 minutes
Sample Preparation~3 min from reagent thaw, No extraction required. 
Sample TypeNP in 3 mL VTM. See Accepted VTMs*
Required Sample Volume42 uL
Storage  (Flux Card) 10° to 30°C, (Flux Reaction Mix) -10° to -30°C
Gene Target gB nd gD 
Format 10 vials, 100 vials, Bulk Plate Reagents 
Reader Compatibility High Throughput thermocycler, Fluxergy Analyzer
Cartridge Compatability8 well strip, PCR plate, Fluxergy Card
Analyzer Size 26.19 cm x 13.13 cm x 25.80 cm 
Analyzer Weight6.8 kg 
Power Input12VDC, 7A
Operating Temperature15° – 30°C 
Storage Temperature15° – 30°C 
Operating Humidity 10% – 85%
Computer Reqs. (Recommended)
OS must be 64-bit, Windows 10+. Intel i5 2.5GHz or equivalent, 8GB DDR4 RAM, HDD: 250 GB, Screen 1080p, USB: 2×2.0 port, Networking: Ethernet port

A Veterinary Tool for Every Equine Facility

horse and vet

On-site testing allows for you to initially prevent spread and react when outbreaks occur:

  • Facilities and Barns
  • Clinics & Referral Hospitals
  • Ambulatory Practices
  • Veterinary Teaching Hospitals
  • Shows
  • Racetracks

Frequently Asked Questions

In recent years, EHV-1 outbreaks at local and FEI shows have had drastic consequences, including numerous horses lost, dozens of show cancellations, and strict quarantines. Because some sectors of the equine community compete throughout the season, multiple shows, barns, and geographic regions can be at risk within a few days.

Yes, EHV-1 has been identified on wood shavings, leather, and other supplies for at least 48 hours, even on items stored outside or in cool temperatures. These findings emphasize the need for increased biosecurity, particularly within high-volume equine-related facilities.

Multiple outbreaks have occurred due to the arrival or return of horses, including clinically healthy horses. Incorporating EHV-1 monitoring–especially for horses traveling from high-risk areas or events–better protects local and international equine populations.

  1. Management of an EHV-1 outbreak at FEI events and its international impact. (2021). The Veterinary Record, 189(5), e905. https://doi.org/10.1002/VETR.905
  2. Saklou, N. T., Burgess, B. A., Ashton, L. V., Morley, P. S., & Goehring, L. S. (2021). Environmental persistence of equid herpesvirus type-1. Equine Veterinary Journal, 53(2), 349–355. https://doi.org/10.1111/EVJ.13313
  3. Friday, P. A., Scarratt, W. K., Elvinger, F., Timoney, P. J., & Bonda, A. (2000). Ataxia and paresis with equine herpesvirus type 1 infection in a herd of riding school horses. Journal of Veterinary Internal Medicine / American College of Veterinary Internal Medicine, 14(2), 197–201. https://doi.org/10.1111/J.1939-1676.2000.TB02236.X
  4. Smith, F. L., Watson, J. L., Spier, S. J., Kilcoyne, I., Mapes, S., Sonder, C., & Pusterla, N. (2018). Frequency of shedding of respiratory pathogens in horses recently imported to the United States. Journal of Veterinary Internal Medicine, 32(4), 1436–1441. https://doi.org/10.1111/JVIM.15145
  5. Irwin, V. L., Traub-Dargatz, J. L., Newton, J. R., Scase, T. J., Davis-Poynter, N. J., Nugent, J., Creis, L., Leaman, T. R., & Smith, K. C. (2007). Investigation and management of an outbreak of abortion related to equine herpesvirus type 1 in unvaccinated ponies. Veterinary Record, 160(11), 378–380. https://doi.org/10.1136/VR.160.11.378

What Experts Say About Fluxergy

"Fluxergy's advance technology can be used on equine patients in order to allow for proper treatment and timely biosecurity. The novel platform can turn a 1 to 3 day turnaround time into an hour processing time."
Dr. Nicola Pusterla, PhD, Dip ACVIM, AVDC
Dr. Nicola Pusterla, PhD, Dip ACVIM, AVDCLeading Equine Infectious Disease Expert​
"Fluxergy is at the forefront of diagnostic capabilities with PCR. The PCR run-time is quick and easy. As we improve our care through newer technologies and diagnostics, such as Fluxergy, we better educate our clients on what is causing the problem."
Dr. Paul Wan, DVM, Dip ACVS, Cert. VBM​
Dr. Paul Wan, DVM, Dip ACVS, Cert. VBM​Veterinarian Practitioner Owner at SoCal Equine Hospital
“I learned of Fluxergy’s technology when I was at Boehringer Ingelheim, and I was impressed about the opportunity for this pointof- care testing platform. I am happy to be involved on Fluxergy’s advisory board where I can bring my 20+ years of animal health and R&D experience. One of the big advantages of Fluxergy’s technology is that it is very easy to use. When veterinarians see horses with high fevers, its important that they can understand quickly what is going on.”
Dr. Randolph Seidler, VMD, MBA
Dr. Randolph Seidler, VMD, MBAFormer global head of R&D and business development for Boehringer Ingelheim Animal Health and Member of Fluxergy’s Advisory Board
“Having the capability to detect Streptococcus equi equi stall-side is an exciting step forward for equine diagnostic testing. The point-of-care PCR platform provides acceptable PCR performance when compared to traditional lab methods and provides an opportunity to save shipping costs and time for veterinarians.”
Dr. Andrew Willis, DVM, DACVIM
Dr. Andrew Willis, DVM, DACVIMBrazos Valley Equine Hospitals
"The ability to offer PCR out of our laboratory opens several new possibilities for our practice, such as a 1 hour test result time for infectious pathogens."
Laura H. Javiscas, VMD, Dip ACVIM
Laura H. Javiscas, VMD, Dip ACVIMRhinebeck Equine, LLP
"Fluxergy's technology has a great potential to start a new era in the diagnostic field. Fluxergy's portable, user-friendly, and cost-effective PCR technology provides highly accurate results in less than an hour, which is needed to control the spread of infectious diseases."
Dr. Lucjan Witkowski, DVM, PhD
Dr. Lucjan Witkowski, DVM, PhDWarsaw University of Life Sciences
"The most exciting thing is that I can turn a stall around in 18 hours and feel very comfortable that my stall is clean and safe to put another horse in. Being able to get that answer quickly is beautiful."
Dr. Ben Buchanan, DVM, DACVIM, DACVECC
Dr. Ben Buchanan, DVM, DACVIM, DACVECCOwner of Brazos Valley Equine Hospitals
"Fluxergy's platform has the capability to change how we handle strangles cases. Being able use PCR to test for S. equi with an hour with a simple workflow will allow me to offer a result immediately to my client and justify where the patient goes or doesn't go. If they can add additional pathogens to create a respriatory panel, then even better. Our practice has been watching Fluxergy's technology grow from an early stage startup and are happy to support innovation that will help our industry."
Dr. Charlie Buchanan
Dr. Charlie BuchananOwner of Signature Equine Hospital
Previous
Next

Fluxergy Resource Library

Download Spec Sheets, Instructions, Troubleshooting Guides, and more. 

Visit the Resource Library

Our Equine Deck

Get to know Fluxergy, our vision, mission, and values. Learn more by downloading our Corporate Deck. 

Download Fluxergy Equine Deck
dr paul wan horse