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Pipetting Techniques

When transferring fluids between steps of our test, proper pipetting techniques are critical to limiting user error. Here are some guidelines to help you with that.

  1. Use Pipette tips with a filtered aerosol barrier.
    This helps to prevent carryover contamination.
  2. Only use tips once.
  3. Pipette slowly and carefully
  4. Ensure that you have pipetted the full volume, whether that is 14 µL or 130 µL.
    Limited sample or reaction mixture volumes can negatively affect your test result.
  5. Avoid pipetting air bubbles
    You can check for air bubbles by visually observing your pipette tip and Sample/Reaction Mixture vials. Air bubbles can adversely affect the performance of microfluidics.
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